Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (132,619)

Singleplex and Multiplex Bead Based Immunoassays (6,174)

ELISA Substrates and Reagents (2,341)

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271 – 285 of 138,521 results

271

Invitrogen™ Human IL-10 ELISA Kit

ELISA

Pricing & Availability
Specifications

Measurement	Absorbance at 450 nm minus absorbance (at 550 nm)
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	Human
Conjugate	HRP
Description	Human IL-10 ELISA Kit
Interassay CV	<10%
Intraassay CV	<10%
Gene Symbol	IL10
Total Assay Time	3 hr
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 50 μL
Assay Sensitivity	<3 pg/mL
Storage Requirements	2-8°C
Assay Range	15.36-600 pg/mL
Test Time	1 hr 20 min
Accession Number	P22301
Regulatory Status	RUO
Target	IL-10
Specificity	No significant cross-reaction with human IL-1 A, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFa, TNFß or IFNg. Approx. 2% cross-reactivity with recombinant mouse IL-10
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Precision	Intra-assay CV <10%; inter-assay CV <10%
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3586
Sample Type	Plasma,Serum,Supernatant,Urine
Label or Dye	Biotin

272

R&D Systems™ Human GDF-15 Quantikine ELISA Kit

Human GDF-15 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Pricing & Availability

273

Invitrogen™ Human TNF alpha ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	7.4%
Intraassay CV	6%
Gene Symbol	Tnf
Total Assay Time	3 hr 10 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 50 μL
Assay Sensitivity	2.3 pg/mL
Storage Requirements	2-8°C
Assay Range	7.8-500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Accession Number	P01375
Regulatory Status	RUO
Target	TNF alpha
Kit Contents	Pre-coated 96 well plate, Standard, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Controls, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Buffer Concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Controls,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	7124
Sample Type	Plasma,Serum,Supernatant,Urine
Label or Dye	Biotin
Synonym	Cachectin, Tumor necrosis factor alpha, DIF

274

R&D Systems™ Mouse/Rat Cystatin C Quantikine ELISA Kit

Mouse/Rat Cystatin C Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Pricing & Availability

275

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R&D Systems™ Human IL-6 Quantikine HS ELISA

Human IL-6 Quantikine HS ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Pricing & Availability

276

Invitrogen™ Human Somatostatin Competitive ELISA Kit

ELISA

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	Human
Conjugate	HRP
Interassay CV	<10%
Intraassay CV	<10%
Gene Symbol	SST
Total Assay Time	2 hr 30 min
Assay Sensitivity	4.69 pg/mL
Storage Requirements	Refer to product documentation for component specific storage temperature.
Assay Range	7.81-500 pg/mL
Test Time	1 hr 20 min
Accession Number	P61278
Regulatory Status	RUO
Target	Somatostatin
Kit Contents	Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer,Standard,Biotinylated Detection Antibody,HRP Conjugate,Standard & Sample Diluent,Biotinylated Detection Antibody Diluent,HRP Conjugate Diluent,Wash Buffer,TMB Substrate,Stop Solution,Plate Sealer
Gene Alias	Antrin; Growth hormone release-inhibiting factor; Neuronostatin; NST; preprosomatostatin; prepro-somatostatin; SMST; SOM; Somatostatin; somatostatin 28; somatostatin-14; Somatostatin-28; somatotropin release-inhibiting factor; SRIF; SS; SS-14; SS-28; Sst; SST-14Antrin; Growth hormone release-inhibiting factor; Neuronostatin; NST; preprosomatostatin; prepro-somatostatin; SMST; SOM; Somatostatin; somatostatin 28; somatostatin-14; Somatostatin-28; somatotropin release-inhibiting factor; SRIF; SS; SS-14; SS-28; Sst; SST-14;
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Gene ID (Entrez)	6750
Sample Type	Cell Lysates,Plasma,Serum,Tissue Homogenates
Label or Dye	Biotin

277

Invitrogen™ Human IFN gamma Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Gene Symbol	IFNG
Total Assay Time	24 hr 30 min
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	8 pg/mL
Storage Requirements	2-8°C
Assay Range	8-500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 45 min
Accession Number	P01579
Regulatory Status	RUO
Target	IFN gamma
Kit Contents	Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Enzyme: Pre-titrated Avidin-HRP,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3458
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin
Synonym	Interferon-gamma, IFN-g, IFNg

278

Invitrogen™ Triglyceride (TG) Colorimetric Assay Kit

These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

Pricing & Availability

279

Invitrogen™ Human HIF1A ELISA Kit

ELISA

Pricing & Availability
Specifications

Measurement	Absorbance at 450 nm minus absorbance (at 550 nm)
Protein Family	Transcription Factors
Immunoassay Kit Format	Sandwich ELISA Kit
Host Species	Human
Target Species	Human,Mouse
Conjugate	HRP
Intraassay CV	<10%
Substrate Type	TMB
Total Assay Time	4 hr
Sample Volume	Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL
Assay Sensitivity	30 pg/mL
Research Discipline	Immunology
For Use With (Application)	Protein Assays and Analysis
Test Time	1 hr 20 min
Detection Method	Colorimetric
Target	HIF1A
Specificity	Specific to human HIF-1 A and shows cross-reactivity with mouse and rat HIF-1 A
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Measuring Range	450 to 550 nm
For Use With (Equipment)	Absorbance Microplate Reader
Label or Dye	Biotin
Interassay CV	<10%
Gene Symbol	HIF1A
Storage Requirements	2-8°C
Assay Range	81.92-20000 pg/mL
Accession Number	Q16665
Regulatory Status	RUO
Precision	Intra-assay CV <10%; inter-assay CV <10%
Product Type	ELISA
Gene ID (Entrez)	3091
Sample Type	Cell Lysates,Plasma,Serum

280

Promotions Available

R&D Systems™ Human VEGF Quantikine ELISA Kit

Human VEGF Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Pricing & Availability
Specifications

Specificity	Natural and Recombinant Human VEGF. This assay also recognizes Recombinant Human VEGF165b.
Target Species	Human
Conjugate	HRP
Gene ID (Entrez)	7422
Sample Type	Cell Culture Supernatant,Plasma (EDTA, Citrate, Heparin),Serum
Sample Volume	Cell Culture Supernates (200uL),Serum (100uL),EDTA Plasma (100uL),Heparin Plasma (100uL),Citrate Plasma (100uL)
Synonym	MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor
Assay Sensitivity	5 pg/mL (Cell Culture Supernates), 9 pg/mL (Serum, Heparin Plasma, Citrate Plasma, EDTA Plasma)
Storage Requirements	Store the unopened product at 2° to 8°C. Do not use past expiration date.
Assay Range	15.60 to 1000 pg/mL (Cell Culture Supernates), 31.30 to 2000 pg/mL (Serum, Heparin Plasma, Citrate Plasma, EDTA Plasma)
For Use With (Application)	ELISA
Cross Reactivity	Cross-species reactivity observed with 1 or more species tested. <0.5% cross-reactivity observed with available related molecules.

281

R&D Systems™ Mouse/Rat FGF-21 Quantikine ELISA Kit

Mouse/Rat FGF-21 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Pricing & Availability

282

Invitrogen™ Mouse IL-6 Uncoated ELISA Kit with Plates

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Gene Symbol	Il6
Total Assay Time	24 hr 30 min
Sample Volume	Cell Lysate 100 μL, Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	8 pg/mL
Storage Requirements	2-8°C
Assay Range	8-500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 45 min
Accession Number	P08505
Regulatory Status	RUO
Target	IL-6
Kit Contents	Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	16193
Sample Type	Cell Lysates,Plasma,Serum,Supernatant
Label or Dye	Biotin
Synonym	Interleukin-6

283

Invitrogen™ Tissue Extraction Reagent I

Lysis buffer for total protein extraction from tissue samples

Pricing & Availability

284

Invitrogen™ Human IgG4 ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	10.2%
Intraassay CV	4.6%
Total Assay Time	1 hr 30 min
Sample Volume	Plasma 20 μL, Serum 20 μL
Assay Sensitivity	0.1 ng/mL
Storage Requirements	2-8°C
Assay Range	9.4-600 ng/mL
Test Time	1 hr 20 min
Regulatory Status	RUO
Target	Human IgG4
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3503
Sample Type	Plasma,Serum
Label or Dye	HRP

285

Invitrogen™ Human IgG (Total) Uncoated ELISA Kit with Plates

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Total Assay Time	24 hr 30 min
Sample Volume	Plasma 10 μL, Serum 10 μL
Assay Sensitivity	1.6 ng/mL
Storage Requirements	2-8°C
Assay Range	1.6-100 ng/mL
For Use With (Application)	ELISA
Test Time	1 hr 45 min
Regulatory Status	RUO
Target	Human IgG
Kit Contents	Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Sample Type	Plasma,Serum
Label or Dye	HRP
Synonym	Immunoglobulin G

Immunoassay Reagents and Kits

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R&D Systems™ Mouse/Rat Cystatin C Quantikine ELISA Kit Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

R&D Systems™ Human IL-6 Quantikine HS ELISA Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

R&D Systems™ Human VEGF Quantikine ELISA Kit Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

R&D Systems™ Mouse/Rat FGF-21 Quantikine ELISA Kit GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

R&D Systems™ Mouse/Rat Cystatin C Quantikine ELISA Kit

R&D Systems™ Human IL-6 Quantikine HS ELISA

R&D Systems™ Human VEGF Quantikine ELISA Kit

R&D Systems™ Mouse/Rat FGF-21 Quantikine ELISA Kit